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rabbit polyclonal antibodies against collagen iv  (OriGene)


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    Structured Review

    OriGene rabbit polyclonal antibodies against collagen iv
    Rabbit Polyclonal Antibodies Against Collagen Iv, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+collagen+iv/Collagen+type+I+Rabbit+Polyclonal+Antibody/pm37446346-1040-14-22
    Average 91 stars, based on 2 article reviews
    rabbit polyclonal antibodies against collagen iv - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Membrane:

    Article Title: Perlecan is critical for heart stability.
    Article Snippet: For determination of the levels of collagen IV and laminin expression, E9.5 hearts were lysed in SDS–Urea sample buffer (8 M Urea, 2 M Thiourea, 0.2% SDS, 1.5% Triton X-100, 0.05 M Tris–HCl, pH 6.8)11 and separated by SDS–PAGE on a 4–15% gradient Tris– HCl gel (Criterion, Bio-Rad). .. Proteins from five hearts each were transferred to PVDF membrane and incubated overnight with a rabbit polyclonal antibody against collagen IV (R1041; 1:500; Acris Antibodies) or a rabbit polyclonal antibody against the laminin IHS (1:1000, kindly provided by M. Paulsson). .. Subsequently, horse radish peroxidase (HRP)-conjugated immunoglobulins (goat antirabbit) and Super Signal West Dura Extended Substrate (Pierce Biotechnology) were used.

    Incubation:

    Article Title: Perlecan is critical for heart stability.
    Article Snippet: For determination of the levels of collagen IV and laminin expression, E9.5 hearts were lysed in SDS–Urea sample buffer (8 M Urea, 2 M Thiourea, 0.2% SDS, 1.5% Triton X-100, 0.05 M Tris–HCl, pH 6.8)11 and separated by SDS–PAGE on a 4–15% gradient Tris– HCl gel (Criterion, Bio-Rad). .. Proteins from five hearts each were transferred to PVDF membrane and incubated overnight with a rabbit polyclonal antibody against collagen IV (R1041; 1:500; Acris Antibodies) or a rabbit polyclonal antibody against the laminin IHS (1:1000, kindly provided by M. Paulsson). .. Subsequently, horse radish peroxidase (HRP)-conjugated immunoglobulins (goat antirabbit) and Super Signal West Dura Extended Substrate (Pierce Biotechnology) were used.



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